231 Collagen type IV bovine
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246 EUR
Bovine collagen type VI isolated from placenta
Source
Bovine placenta
Purity
Bovine collagen type VI 90%
Format
Lyophilized, salt-free
Purification
Collagen was extracted from washed dissected tissue into dilute acetic acid after pepsin treatment. Collagen type VI was purified by using differential salt precipitation
Applications
ELISA, Immunoprecipitation, Western Blotting
Reconstitution
Collagen type VI dissolves very slowly in 0.5 M acetic acid and requires both vigorous mixing and time.
Add small amount of 0.5 M acetic acid, pH 2.5 and use Potter homogenizer (or similar) to break up the protein cake into suspension. Add 0.5 M acetic acid, pH 2.5 to yield 0.1 mg/ml concentration and use vigorous stirring for several days.
Concentration higher than 0.1 mg/ml in acetic acid requires even longer time. Use 6M urea (or other chaotropic agent) if application allows it.
Storage
Lyophilized protein: shipping at ambient temperature, long term storage (2 years) at -20°C or lower. Storage of reconstituted protein 1 month at +4°C
