231 Collagen type IV bovine

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Article Number: 231

Bovine collagen type VI isolated from placenta


Catalog number 231

Amount 1 mg

Source

Bovine placenta


Purity

Bovine collagen type VI 90%


Format

Lyophilized, salt-free


Purification

Collagen was extracted from washed dissected tissue into dilute acetic acid after pepsin treatment. Collagen type VI was purified by using differential salt precipitation


Applications

ELISA, Immunoprecipitation, Western Blotting


Reconstitution

Collagen type VI dissolves very slowly in 0.5 M acetic acid and requires both vigorous mixing and time.

Add small amount of 0.5 M acetic acid, pH 2.5 and use Potter homogenizer (or similar) to break up the protein cake into suspension. Add 0.5 M acetic acid, pH 2.5 to yield 0.1 mg/ml concentration and use vigorous stirring for several days.

Concentration higher than 0.1 mg/ml in acetic acid requires even longer time. Use 6M urea (or other chaotropic agent) if application allows it.

Storage


Lyophilized protein: shipping at ambient temperature, long term storage (2 years) at -20°C or lower. Storage of reconstituted protein 1 month at +4°C