425 Collagen type IV (human) 0.1 mg
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564 EUR
Human collagen type IV purified from placenta
Source
Human placenta. Negative for HBsAg, HCV, HIV1 and HIV2
Purity
Human collagen type IV 95%. Other human collagens below 5%. Non-collagen proteins below 0.5%
Format
Lyophilized, salt-free
Purification
Partial pepsin digestion in acidic conditions and differential salt precipitation
Applications
Standard for immunological assays. Antigen for antibody production. Coating material for cell culture studies
Standard for ELISA and dot blotting (1)
Collagen type IV was used to polarize THP1 monocytes differentiation (2)
Collagen type IV was used at 250 ug/ml for cell attachment in conjugated ECM-PA microarrays (3)
Collagen type IV was used for fabrication of cell microarrays that were used for studying drug response on lung adenocarcinoma cells (4)
Exogenous collagen IV rescues dying siNotch3 ovarian cancer cells (5)
Human adipose-derived stem cells (ASCs) culture on human collagen type IV (7)
Use 0.5 M acetic acid, pH 2.5
Storage
Shipping at ambient temperature. Storage 2 years at -20°C or lower
Citations
1. Kuzan A, Chwilkowska A, Maksymowicz K, Szydelko-Bronowicka A, Stach K, Pezowicz C, Gamian A (2018) Advanced glycation end products as a source of artifacts in immunoenzymatic methods. Glycoconjugate Journal 35(1) DOI: 10.1007/s10719-017-9805-4
2. Pal S, V. Badireenath Konkimalla (2016) Data on sulforaphane treatment mediated suppression of autoreactive, inflammatory M1 macrophages. Data in Brief. https://doi.org/10.1016/j.dib.2016.03.105
3. Kourouklis AP, Kaylan KB, Underhill GH (2016) Substrate stiffness and matrix composition coordinately control the differentiation of liver progenitor cells. Biomaterials 99:82-94
4. Kaylan KB, Gentile SD, Milling LE, Bhinge KN, Kosari F and Underhill GH (2016) Mapping lung tumor cell drug responses as a function of matrix context and genotype using cell microarrays. Integrative Biology. Issue 12
5. Brown CW, Brodsky AS and Freiman RN (2015) Notch3 Overexpression Promotes Anoikis Resistance in Epithelial Ovarian Cancer via Upregulation of COL4A2. Mol. Cancer Res. vol 13, issue 1
6. Kaylan KB, Kourouklis AP, Underhill GH A High-throughput Cell Microarray Platform for Correlative Analysis of Cell Differentiation and Traction Forces. https://www.jove.com/video/55362
7. Clevenger TN, Hinman CR, Ashley Rubin RK, Smither K, Burke DJ, Hawker CJ, Messina D, van Epps D, Clegg DO (2016) Vitronectin-Based, Biomimetic Encapsulating Hydrogel Scaffolds Support Adipogenesis of Adipose Stem Cells. Tissue Engineering Part A. 22:7-8
