425 Collagen type IV (human) 0.1 mg

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Article Number: 425

Human collagen type IV purified from placenta

Source

Human placenta. Negative for HBsAg, HCV, HIV1 and HIV2


Purity

Human collagen type IV 95%. Other human collagens below 5%. Non-collagen proteins below 0.5%


Format

Lyophilized, salt-free


Purification

Partial pepsin digestion in acidic conditions and differential salt precipitation


Applications

Standard for immunological assays. Antigen for antibody production. Coating material for cell culture studies

Standard for ELISA and dot blotting (1)

Collagen type IV was used  to polarize THP1 monocytes differentiation (2)

Collagen type IV was used at 250 ug/ml for cell attachment in conjugated ECM-PA microarrays (3)

Collagen type IV was used  for fabrication of cell microarrays that were used for studying drug response on lung adenocarcinoma cells (4)

Exogenous collagen IV rescues dying siNotch3 ovarian cancer cells (5)


Human adipose-derived stem cells (ASCs) culture on human collagen type IV (7)


Reconstitution

Use 0.5 M acetic acid, pH 2.5


Storage

Shipping at ambient temperature. Storage 2 years at -20°C or lower


Citations

1. Kuzan A, Chwilkowska A, Maksymowicz K, Szydelko-Bronowicka A, Stach K, Pezowicz C, Gamian A (2018) Advanced glycation end products as a source of artifacts in immunoenzymatic methods. Glycoconjugate Journal 35(1) DOI: 10.1007/s10719-017-9805-4


2. Pal S, V. Badireenath Konkimalla (2016) Data on sulforaphane treatment mediated suppression of autoreactive, inflammatory M1 macrophages. Data in Brief. https://doi.org/10.1016/j.dib.2016.03.105


3. Kourouklis AP, Kaylan KB, Underhill GH (2016) Substrate stiffness and matrix composition coordinately control the differentiation of liver progenitor cells. Biomaterials 99:82-94


4. Kaylan KB, Gentile SD, Milling LE, Bhinge KN, Kosari F and Underhill GH (2016) Mapping lung tumor cell drug responses as a function of matrix context and genotype using cell microarrays. Integrative Biology. Issue 12


5. Brown CW, Brodsky AS and Freiman RN (2015) Notch3 Overexpression Promotes Anoikis Resistance in Epithelial Ovarian Cancer via Upregulation of COL4A2. Mol. Cancer Res. vol 13, issue 1


6. Kaylan KB, Kourouklis AP, Underhill GH A High-throughput Cell Microarray Platform for Correlative Analysis of Cell Differentiation and Traction Forces.  https://www.jove.com/video/55362


7. Clevenger TN, Hinman CR, Ashley Rubin RK, Smither K, Burke DJ, Hawker CJ, Messina D, van Epps D, Clegg DO (2016) Vitronectin-Based, Biomimetic Encapsulating Hydrogel Scaffolds Support Adipogenesis of Adipose Stem Cells. Tissue Engineering Part A. 22:7-8