169 Collagen type III (bovine) 10 mg
0 EUR
137 EUR
Out of stock
NOTE: This product is replaced by #576 (click here)
Bovine collagen type III isolated from calf skin
Source
Bovine (calf) skin. Collagen was extracted from washed dissected tissue into dilute acetic acid. Collagen type III was purified by partial pepsin digestion in acidic conditions and differential salt precipitation
Purity
Bovine collagen type III 90%. Other bovine collagen types 10%. Non-collagen proteins below 0.5%
Format
Lyophilized, salt-free
Purification
Partial pepsin digestion in acidic conditions and differential salt precipitation
Applications
Bovine collagen (type III) standard. Antigen for antibody production. Coating material for cell culture studies. Collagen microfibrils will activate platelets causing aggregation. May not be suitable for 3-D gel formation
Reconstitution
Use 0.5 M acetic acid, pH 2.5. Dissolved collagen retains immunologic properties of native collagen. Structure of native collagen confirmed by ability to form microfibrils
Storage
Lyophilized protein: shipping at ambient temperature, long term storage (2 years) at -20°C or lower. Storage of reconstituted protein 1 month at +4°C