169 Collagen type III (bovine) 10 mg

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Article Number: 169

NOTE: This product is replaced by #576 (click here)


Bovine collagen type III isolated from calf skin

Source

Bovine (calf) skin. Collagen was extracted from washed dissected tissue into dilute acetic acid. Collagen type III was purified by partial pepsin digestion in acidic conditions and differential salt precipitation


Purity

Bovine collagen type III 90%. Other bovine collagen types 10%. Non-collagen proteins below 0.5%


Format

Lyophilized, salt-free


Purification

Partial pepsin digestion in acidic conditions and differential salt precipitation


Applications

Bovine collagen (type III) standard. Antigen for antibody production. Coating material for cell culture studies. Collagen microfibrils will activate platelets causing aggregation. May not be suitable for 3-D gel formation


Reconstitution

Use 0.5 M acetic acid, pH 2.5. Dissolved collagen retains immunologic properties of native collagen. Structure of native collagen confirmed by ability to form microfibrils


Storage

Lyophilized protein: shipping at ambient temperature, long term storage (2 years) at -20°C or lower. Storage of reconstituted protein 1 month at +4°C