221 Collagen type I and III (mouse) 5 mg
0 EUR
345 EUR
Mouse collagen type I + III (murine tail-tendon atelocollagen), 5 mg
Pepsin-solubilised mixture of mouse collagen type I and type III (about 45% each) from tail tendon, lyophilised salt-free, 5 mg. A species-matched collagen for mouse work: standard for ELISA and Western blotting of murine collagen, antigen and positive control when validating anti-collagen antibodies on mouse tissue, and coating substrate for murine cell culture. Not intended for gel formation; for sterile gel-grade mouse collagen I see cat. 603, for pure mouse collagen I see cat. 342.
Source
Mouse tail tendons
Purity
Murine collagens: type I 45%, type III 45%, type IV 10%, type V below 1%. Non-collagen proteins below 0.5%
Format
Lyophilized, salt-free
Purification
Partial pepsin digestion in acidic conditions and differential salt precipitation
Applications
Standard for immunological assays. Coating material for cell culture studies. May not be suitable for 3-D gel formation
Reconstitution
Use 0.05-0.5 M acetic acid, pH 2.5 at 4°C. Dissolved collagen retains immunologic properties of native collagen. Structure of native collagen confirmed by ability to form microfibrils
Storage
Shipping at ambient temperature. Storage of lyophilized protein 2 years at -20°C. Storage of reconstituted protein 1 month at +4°C
