221 Collagen type I and III (mouse) 5 mg

In Stock

195 USD

Article Number: 221

Murine collagen type I and III purified from tail tendons of the mouse


Source

Mouse tail tendons


Purity

Murine collagens: type I 45%, type III 45%, type IV 10%, type V below 1%. Non-collagen proteins below 0.5%


Format

Lyophilized, salt-free


Purification

Partial pepsin digestion in acidic conditions and differential salt precipitation


Applications

Standard for immunological assays. Coating material for cell culture studies. May not be suitable for 3-D gel formation


Reconstitution

Use 0.05-0.5 M acetic acid, pH 2.5 at 4°C. Dissolved collagen retains immunologic properties of native collagen. Structure of native collagen confirmed by ability to form microfibrils


Storage

Shipping at ambient temperature. Storage of lyophilized protein 2 years at -20°C. Storage of reconstituted protein 1 month at +4°C