436 Collagen type V (human) 0.1 mg

Human collagen type V purified from placenta

Source

Human placenta. Negative for HBsAg, HCV, HIV1 and HIV2

Purity

Human collagen type V at least 95%, other human collagens below 5%, non-collagen proteins below 1%.

Format

Lyophilized.

Purification

Partial pepsin digestion in acidic conditions and differential salt precipitation

Applications

Type V collagen standard (1, 2, 3), Antigen for antibody production, Coating material for cell culture studies

Detection of IgGs against disc ECM proteins by immunoblotting (1)

Reconstitution

Use 0.1 M acetic acid, pH 3.0

Storage

Shipping at ambient temperature. Storage 2 years at -20°C or lower

Citations

1. Capossela S, Schläfli P, Bertolo A, Janner T, Stadler BM, Pätzel T, Baur M, Stoyanov JV. Degenerated human intervertebral discs contain autoantibodies against extracellular matrix proteins. Eur Cell Mater. 2014 Apr 4;27:251-63

2. Philips N, Chalensouk-Khaosaat J and Gonzalez S (2015) Stimulation of the Fibrillar Collagen and Heat Shock Proteins by Nicotinamide or Its Derivatives in Non-Irradiated or UVA Radiated Fibroblasts, and Direct Anti-Oxidant Activity of Nicotinamide Derivatives. Cosmetics 2015, 2(2), 146-161; doi:10.3390/cosmetics2020146

3. Philips N, Samuel P, Parakandi H, Gopal S, Siomyk H, Ministro A, Thompson T, Borkow G (2012) Beneficial Regulation of Fibrillar Collagens, Heat Shock Protein-47, Elastin Fiber Components, Transforming Growth Factor 1, Vascular Endothelial Growth Factor and Oxidative Stress Effects by Copper in Dermal Fibroblasts. Connective Tissue Research 1-7