184 Collagen type V (bovine, atelocollagen, sterile, lyophilized)
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363 EUR
Tuotenumero: 184
Bovine collagen type V atelocollagen, sterile, 1 mg – minor fibrillar collagen for coating
Highly purified (>98%) type V collagen from bovine placenta, pepsin-solubilised and supplied sterile, lyophilised and salt-free. Collagen V is the minor fibrillar collagen that co-assembles with type I and regulates fibril diameter in cornea, skin, bone and placenta. The sterile 1 mg pack is intended for coating culture surfaces in adhesion and migration studies, as a substrate for integrin-binding experiments and as a type V standard or antigen. The pepsin-treated collagen does not form stable gels on its own.
Catalog Number 184
Amount 1 mg
Source
Bovine placenta
Purity
Bovine collagen type V > 98%
Format
Lyophilized, salt-free
Purification
Collagen was extracted from washed dissected tissue into dilute acetic acid after mild pepsin treatment. Collagen type V was purified by using differential salt precipitation
Sterility
Passed sterility test for bacteria and fungi
Applications
Cell culture (coating)
Reconstitution
Dissolve in 20 mM acetic acid. Suspension should be shaken or stirred for several hours or overnight between 2-8°C. Material is dissolved when it appears homogeneous, with no schlieren pattern (light refraction due to differing protein concentrations in the suspension). Recommended concentration 1-5 mg/ml. After dissolution store 1 month at +4°C
Storage
Lyophilized protein: shipping at ambient temperature, long term storage (2 years) at -20°C or lower. Storage of reconstituted protein 1 month at +4°C
RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, DRUG OR HOUSEHOLD USE
