677 Goat anti-human IgA polyclonal antibody FITC 1.2 mg

Polyclonal goat antibody anti-human IgA conjugated to FITC (cross-adsorbed and affinity purified)

Specificity

Human immunoglobulin IgA. No reactivity against human IgG, IgM, other serum proteins

Preparation

Purification by affinity chromatography using immobilized IgA. Cross-adsorption against human IgG, IgM and other serum proteins. Chromatographic separation of free fluorescein and protein aggregates. Labeling four molecules FITC per antibody

Format

Antibody solution 1.2 mg/ml in the buffer (0.05M sodium phosphate pH 7.5, 0.1 M NaCl) containing 50% glycerol and 5 mg/ml bovine serum albumin

Applications and recommended dilutions

Western blotting, ELISA, Immunohistochemistry IHC (1:200), Cell staining, Cell sorting, Electron microscopy

Storage

Shipping at ambient temperature. Storage at -20°C for 2 years

Citations

1) Hecker J, Diethers A, Schulz D, Sabri A, Plum M, Michel Y, Mempel M, Ollert M, Jakob T, Blank S, Braren I, Spillner E. (2012) An IgE epitope of Bet v 1 and fagales PR10 proteins as defined by a human monoclonal IgE. Allergy. 2012 Dec;67(12):1530-7 (PMID: 23066955)

2) Hecker J, Diethers A, Etzold S, Seismann H, Michel Y, Plum M, Bredehorst R, Blank S, Braren I, Spillner E. (2011) Generation and epitope analysis of human monoclonal antibody isotypes with specificity for the Timothy grass major allergen Phl p 5a. Mol Immunol. 2011 May;48(9-10):1236-44. (PMID: 21474184)

3) Hecker J (2011) Charakterisierungen originörer IgE-Epitope der Majorallergene Bet v 1 und Phl p 5 durch Interaktionsanalysen mit rekombinanten humanen Antikörpern. PhD dissertation. Universität Hamburg