882 Collagen type I (porcine, native collagen suitable for 3D gel, sterile, lyophilized)
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709 EUR
Tuotenumero: 882
Description
Collagen type I protein purified from porcine tendon (native collagen suitable for making 3D gel). Sterile and lyophilized
Catalog Number 882
Amount 25 mg
Source
Porcine tendons
Purity
Porcine collagen type I > 90%, porcine collagen type III < 10%
Format
Lyophilized, salt-free
Purification
Collagen was extracted from washed dissected tissue into dilute acetic acid. Collagen type I was purified by using differential salt precipitation
Sterility
Passed sterility test for bacteria and fungi
Applications
Cell culture (coating or 3D gel)
Reconstitution
Dissolve in 20 mM acetic acid at collagen concentration 5 mg/ml. Suspension should be shaken or stirred for several hours or overnight between 2-8°C. Material is dissolved when it appears homogeneous, with no schlieren pattern (light refraction due to differing protein concentrations in the suspension). Recommended concentration 1-5 mg/ml. After dissolution store 1 month at +4C.
Storage
Lyophilized protein: shipping at ambient temperature, long term storage (2 years) at -20°C or lower. Storage of reconstituted protein 1 month at +4°C
RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, DRUG OR HOUSEHOLD USE
