467 Rabbit anti-human IgG polyclonal antibody HRP 1.2 mg

Polyclonal rabbit antibody anti-human immunoglobulin IgG conjugated to HRP (affinity-purified and cross-adsorbed)

Specificity

Human immunoglobulin IgG. No cross-reactivity to human IgM, IgA and other human plasma proteins

Preparation

Purification by affinity chromatography using immobilized human IgG. Cross-adsorption with immobilized human IgM and IgA. Purification by gel filtration and anion-exchange chromatography to remove protein aggregates and free peroxidase

Format

Antibody solution 1.2 mg/ml in the buffer (0.05M sodium phosphate pH 7.5, 0.1 M NaCl, 50% glycerol, 5 mg/ml BSA)

Applications and recommended dilutions

ELISA (x1000), Immunohistology, Blotting. Dilution should be adjusted to the appropriate task. Do not use azide together with HRP-conjugated products since azide strongly inhibits horseradish peroxidase (HRP)

Storage

Shipping at ambient temperature. Storage for 1 year at -20°C or lower

Citations

1. Kleinschmidt M, Schoenfeld R, Göttlich C, Bittner D, Metzner JE, Leplow B, Demuth H-U (2016) Characterizing Aging, Mild Cognitive Impairment, and Dementia with Blood-Based Biomarkers and Neuropsychology. Journal of Alzheimer's Disease 50:111-126