201 Rabbit anti-human collagen type V polyclonal antibody 0.5 ml
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492 EUR
Rabbit anti-human collagen type V polyclonal antibody (COL5A1), cross-adsorbed, 0.5 ml
Rabbit polyclonal raised against native human placental collagen type V and made type-specific by affinity purification and cross-adsorption on human collagens I, II, III and IV and plasma proteins. Type V is a minor fibrillar collagen enriched in cornea, placenta, blood-vessel walls and fibrotic tissue; this antibody stains it in frozen human sections (~1:20) and quantifies it in ELISA (~1:3000) — relevant for corneal, fibrosis, transplant-rejection (anti-collagen V autoimmunity) and matrix-assembly research. Lyophilised; reconstitute with 0.5 ml water.
Specificity
Binds human Collagen type V. No cross-reactivity to other human collagens. No cross-reactivity to human plasma proteins
Preparation
Affinity purification on immobilized Collagen type V with cross-absorption to immobilised human blood plasma proteins and human collagen type I, II, III and IV.
Format
Lyophilized from 0.01M sodium phosphate, 0.01M sodium borate, 0.15M sodium chloride, 1% dextran, 1% mannitol, pH 8
Applications and recommended dilutions
ELISA (x3000), Histochemistry (frozen sections) (x20). Not suitable for denaturing and dissociating conditions
Reconstitution
Use 0.5 ml sterile pure water
Storage
Shipping at ambient temperature. Storage of lyophilized antibodies for 2 years at -20°C or lower
