807 Mouse anti-human IgA monoclonal antibody, clone BFG-267 1 ml
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321 EUR
Mouse anti-human IgA (α-chain) monoclonal antibody, clone BFG-267, 1 ml
Mouse IgG1 monoclonal directed against the α heavy chain of human IgA, with no reactivity to IgG, IgM, IgE or other plasma proteins. Purified from ascites by sodium-sulphate precipitation and DEAE chromatography and lyophilised (reconstitute with 1 ml water). A class-specific reagent for human IgA ELISA (~1:1000) and for immunohistochemistry (~1:40) of IgA plasma cells in gut, salivary gland and tonsil — useful in IgA nephropathy, coeliac and mucosal-immunity research and as the monoclonal partner to our polyclonal anti-IgA antibodies (cat. 395, 541).
Specificity
Specific against human IgA, a-chain. No reactivity to human IgE, IgG, IgM or other human plasma proteins
Preparation
Precipitation by sodium sulphate and anion-exchange chromatography (DEAE-cellulose) with gradient elution
Format
Lyophilized from 0.01M sodium phosphate, 0.01M sodium borate, 0.15M sodium chloride, 1% dextran, 1% mannitol, pH 8
Applications and recommended dilutions
ELISA (x1000), Immunohistochemistry (x40)
Reconstitution
Use 1 ml sterile pure water, aliquot and store at -20°C or lower.
Storage
Shipping at ambient temperature. Storage of lyophilized antibodies 2 years at -20°C or lower
