168 Rabbit anti-human urokinase polyclonal antibody 0.5 mg
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348 EUR
Polyclonal rabbit antibody anti-human urokinase (abbokinase, urokinase-type Plasminogen Activator, uPA, PLAU)
Specificity
The antibody binds to human HMW-scuPA (54 kDa), HMW-tsuPA (52 kDa) and LMW-scuPA (33 kDa) with high apparent affinity (Kd= 0.1 nM) completely inhibiting the ability of urokinase to activate plasminogen. Urokinase reaction with synthetic substrate S2444 is also inhibited. The antibody is highly specific for urokinase, no cross reactions were found with other serine proteases (tissue plasminogen activator, plasmin, trypsin, chymotrypsin, elastase, thrombin). Human whole plasma or plasma proteins do not interfere with its binding to urokinase
Preparation
Affinity purification with immobilized human urokinase. Cross-adsorption on immobilised human plasma proteins
Format
Lyophilized from 0.01M sodium phosphate, 0.01M sodium borate, 0.15M sodium chloride, 1% dextran, 1% mannitol, pH 8
Applications and recommended dilutions
ELISA (x5000), IHC (x50)
Reconstitution
Use 1 ml sterile pure water
Storage
Shipping at ambient temperature. Storage of lyophilized antibodies 2 years at -20°C or lower
