168 Rabbit anti-human urokinase polyclonal antibody 0.5 mg

In Stock

315 USD

Article Number: 168

Polyclonal rabbit antibody anti-human urokinase (abbokinase, urokinase-type Plasminogen Activator, uPA, PLAU)


Specificity

The antibody binds to human HMW-scuPA (54 kDa), HMW-tsuPA (52 kDa) and LMW-scuPA (33 kDa) with high apparent affinity (Kd= 0.1 nM) completely inhibiting the ability of urokinase to activate plasminogen. Urokinase reaction with synthetic substrate S2444 is also inhibited. The antibody is highly specific for urokinase, no cross reactions were found with other serine proteases (tissue plasminogen activator, plasmin, trypsin, chymotrypsin, elastase, thrombin). Human whole plasma or plasma proteins do not interfere with its binding to urokinase


Preparation

Affinity purification with immobilized human urokinase. Cross-adsorption on immobilised human plasma proteins


Format

Lyophilized from 0.01M sodium phosphate, 0.01M sodium borate, 0.15M sodium chloride, 1% dextran, 1% mannitol, pH 8

Applications and recommended dilutions

ELISA (x5000), IHC (x50)


Reconstitution

Use 1 ml sterile pure water


Storage

Shipping at ambient temperature. Storage of lyophilized antibodies 2 years at -20°C or lower