173 Sheep anti-rabbit IgG, IgM, IgA polyclonal antibody FITC 1.2 mg
0 EUR
287 EUR
Polyclonal sheep antibody anti-rabbit immunoglobulins IgG, IgM, IgA conjugated to FITC
Specificity
Binds rabbit immunoglobulins IgG, IgM, IgA. Affinity to human IgM, IgG, IgA as measured by ELISA below 3%
Preparation
Purification by affinity chromatography using antigen coupled to sorbent. Antibody concentration was determined by extinction coefficient prior to conjugation to fluorescein isothiocyanate (FITC). Chromatographic separation of free fluorescein and protein aggregates. Labeling four molecules FITC per antibody
Format
Solution 1.2 mg/mL antibody, 0.05 M sodium phosphate pH 7.5, 0.1 M NaCl, 50% glycerol, 5 mg/ml BSA
Applications and recommended dilutions
Western blotting, ELISA, Immunohistology (1:200), Cell staining, Cell sorting, Electron microscopy
Storage
Shipping at ambient temperature. Storage at -20°C for 2 years
Citations
1. Ehlicke, F., Freimark, D., Heil, B., Dorresteijn, A., Czermak, P. (2010). Intervertebral disc regeneration: Influence of growth factors on differentiation of human mesenchymal stem cells (hMSC). International Journal of Artificial Organs, 33 (4), 244-252.
2. Lee E, Choi J, Lee HS (2017) Palmitate induces mitochondrial superoxide generation and activates AMPK in podocytes. J. Cellular Physiology 232:12:3209-3217
