173 Sheep anti-rabbit IgG, IgM, IgA polyclonal antibody FITC 1.2 mg

In Stock

225 USD

Article Number: 173

Polyclonal sheep antibody anti-rabbit immunoglobulins IgG, IgM, IgA conjugated to FITC


Specificity

Binds rabbit immunoglobulins IgG, IgM, IgA. Affinity to human IgM, IgG, IgA as measured by ELISA below 3%


Preparation

Purification by affinity chromatography using antigen coupled to sorbent. Antibody concentration was determined by extinction coefficient prior to conjugation to fluorescein isothiocyanate (FITC). Chromatographic separation of free fluorescein and protein aggregates. Labeling four molecules FITC per antibody


Format

Solution 1.2 mg/mL antibody, 0.05 M sodium phosphate pH 7.5, 0.1 M NaCl, 50% glycerol, 5 mg/ml BSA


Applications and recommended dilutions

Western blotting, ELISA, Immunohistology (1:200), Cell staining, Cell sorting, Electron microscopy


Storage

Shipping at ambient temperature. Storage at -20°C for 2 years


Citations 

1. Ehlicke, F., Freimark, D., Heil, B., Dorresteijn, A., Czermak, P. (2010). Intervertebral disc regeneration: Influence of growth factors on differentiation of human mesenchymal stem cells (hMSC). International Journal of Artificial Organs, 33 (4), 244-252.

2. Lee E, Choi J, Lee HS (2017) Palmitate induces mitochondrial superoxide generation and activates AMPK in podocytes. J. Cellular Physiology 232:12:3209-3217