136 Mouse anti-human CD31 (Platelet GPIIa', PECAM1) monoclonal antibody, clone VM64 1 ml
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363 EUR
Mouse anti-human CD31 (PECAM-1) monoclonal antibody, clone VM64, 1 ml
Mouse IgG2bκ monoclonal antibody against CD31 / PECAM-1, the 140 kDa adhesion molecule concentrated at endothelial cell–cell junctions and expressed on platelets, monocytes, granulocytes and B cells. It outlines endothelial cell borders with a silver-stain-like pattern, making it a classic endothelial and vessel marker. Purified IgG, lyophilised, 1 ml after reconstitution; for immunohistochemistry, immunofluorescence, flow cytometry, non-reducing immunoblot and functional studies of endothelial monolayer integrity.
Specificity
The monoclonal antibody is directed against the CD31 antigen, a 140 kD protein, which is expressed on human platelets and endothelial cells and is designated as PECAM-1 (platelet-endothelial cell adhesion molecule). The antibody reacts with platelets, monocytes/macrophages, granulocytes and B-cells. It stains the cell boundaries of endothelial cells with a staining pattern identical to that of silver staining. Furthermore in histology it stains weakly histiocytes and glomeruli
Preparation
Ammonium sulphate precipitation and DEAE-cellulose chromatography with gradient elution
Format
Purified and lyophilized. Contains azide and PBS. The azide concentration in reconstituted solution is 3 mM
Applications
Functional studies on cell-cell interactions. Studies on integrity of endothelial cell monolayers. The antibody can be used for immunoblotting under non reducing conditions. The antibody is useful for indirect immunofluorescence staining with analysis by cytofluorometry or fluorescence microscopy and for immunohistochemistry.
Reconstitution
Use 1 ml sterile pure water
Storage
Shipping at ambient temperature. Storage of lyophilized antibodies 2 years at -20°C or lower
