192 Monoclonal antibody anti-human CD36 (Platelet GPIV, Thrombospondin receptor) 1 ml
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363 EUR
Mouse anti-human CD36 (platelet GPIV, thrombospondin receptor) monoclonal antibody, clone VM58, 1 ml
Mouse IgG1κ monoclonal to CD36, the 88-90 kDa class B scavenger receptor on platelets, monocytes/macrophages, erythroblasts, microvascular endothelium and adipocytes that binds thrombospondin-1, collagen, oxidised LDL and long-chain fatty acids. Clone VM58 is a functional antibody: it triggers FcγRII-dependent platelet aggregation and is used in platelet-activation and CD36-deficiency (Naka-negative) studies, in indirect immunofluorescence and flow cytometry of blood cells, and in immunohistochemistry. Purified, lyophilised; reconstitute with 1 ml water.
Specificity
The monoclonal antibody is directed against the CD36 antigen (GP IV, GP IIIa, Thrombospondin receptor) (90 kDa), which is expressed on human platelets. Antibody reacts with platelets, monocytes, macrophages, erythroblasts and weakly with B-cells. In immunohistology the antibody reacts with some endothelial cells, adipocytes and the granular layer of the skin. Antibody induces Fc-receptor-dependent platelet aggregation after binding to platelet. The heterogeneity of the response found in healthy individuals is dependent on the polymorphism of the Fc RII on platelets
Preparation
Ammonium sulphate precipitation and DEAE-cellulose chromatography with gradient elution
Format
Purified and lyophilized from PBS with sodium azide. The azide concentration in reconstituted solution is 3 mM
Applications
Functional studies. Indirect immunofluorescence. Immunohistochemistry.
For fluorescence technique on cells fixation with 1% paraformaldehyde for 5 minutes is advised. Use for WB is not recommended
Reconstitution
Use 1 ml sterile pure water
Storage
Shipping at ambient temperature. Storage of lyophilized antibodies 2 years at -20°C or lower
References
Gardiner EE, Karunakaran D, Arthur JF, Mu FT, Powell MS, Baker RI, Hogarth PM, Kahn ML, Andrews RK, Berndt MC. (2008) Dual ITAM-mediated proteolytic pathways for irreversible inactivation of platelet receptors: de-ITAM-izing FcgammaRIIa. Blood. 111(1):165-174
Burgess JK, Lindeman R, Chesterman CN, Chong BH. (1995) Single amino acid mutation of Fc gamma receptor is associated with the development of heparin-induced thrombocytopenia. Br J Haematol. 91(3):761-766
Mazurov AV, Vinogradov DV, Vlasik TN, Burns GF, Berndt MC. (1992) Heterogeneity of Platelet Fc-receptor-dependent Response to Activating Monoclonal Antibodies. Platelets. 3(4):181-188
Vinogradov DV, Vlasik TN, Agafonova OG, Vasil'ev SA, Lagutina NIa, Makarov VA, Berndt M, Mazurov AV. (1991) [Inhibition of Fc-receptor dependent platelet aggregation by monoclonal antibodies against the glycoprotein IIb-IIIa complex]. Biokhimiia. 56(5):787-797
