739 Collagen type I and III (canine) 10 mg

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287 EUR

Article Number: 739

Canine collagen type I and III


Canine tissue. Collagen was extracted from washed dissected tissue into dilute acetic acid after pepsin treatment. Collagen type I and III was purified by using differential salt precipitation


Canine collagen I and III ヨ 98%, canine collagen (other types) <1%, non-collagen proteins <0.5%


Lyophilized, salt-free


Partial pepsin digestion in acidic conditions and differential salt precipitation


Coating material for cell culture studies. May not be suitable for 3-D gel formation


Use 0.5 M acetic acid, pH 2.5. Mix vigorously overnight and use homogenizer if required to disperse the recalcitrant sediment. Dissolved collagen retains immunologic properties of native collagen. Structure of native collagen confirmed by ability to form microfibrils


Shipping at ambient temperature. Storage of lyophilized protein 2 years at -20°C. Storage of reconstituted protein 1 month at +4°C


1. Ferkau A, Gillmann HJ, Mischke R, Calmer S, Ecklebe S, Abid M, Minde JW, Echtermeyer F, Theilmeier G. (2013) Infection-associated platelet dysfunction of canine platelets detected in a flow chamber model. BMC Vet Res. 2013 Jun 7;9(1):112 (PMID: 23758817)

2. Ferkau A, Ecklebe S, Jahn K, Calmer S, Theilmeier G, Mischke R. (2013) A dynamic flow-chamber-based adhesion assay to assess canine platelet-matrix interactions in vitro. Vet Clin Pathol. 2013 Jun;42(2):150-6 (PMID: 23627964)