739 Collagen type I and III (canine) 10 mg
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363 EUR
Canine (dog) collagen type I + III, pepsin-solubilised atelocollagen, 10 mg
Mixed type I and III collagen purified from dog tissue by pepsin solubilisation and salt fractionation (≥98% collagen I + III), lyophilised salt-free. A species-specific collagen for veterinary and comparative research: standard and antigen for canine collagen immunoassays and for validating anti-collagen antibodies on dog tissue, and a coating substrate for canine cell culture. Not intended for 3D gel formation. The matching affinity-purified antibody is cat. 775 (rabbit anti-canine collagen I/III).
Source
Canine tissue. Collagen was extracted from washed dissected tissue into dilute acetic acid after pepsin treatment. Collagen type I and III was purified by using differential salt precipitation
Purity
Canine collagen I and III ≥98%, canine collagen (other types) <1%, non-collagen proteins <0.5%
Format
Lyophilized, salt-free
Purification
Partial pepsin digestion in acidic conditions and differential salt precipitation
Applications
Coating material for cell culture studies. May not be suitable for 3-D gel formation
Reconstitution
Use 0.5 M acetic acid, pH 2.5. Mix vigorously overnight and use homogenizer if required to disperse the recalcitrant sediment. Dissolved collagen retains immunologic properties of native collagen. Structure of native collagen confirmed by ability to form microfibrils
Storage
Shipping at ambient temperature. Storage of lyophilized protein 2 years at -20°C. Storage of reconstituted protein 1 month at +4°C
Publications
1. Ferkau A, Gillmann HJ, Mischke R, Calmer S, Ecklebe S, Abid M, Minde JW, Echtermeyer F, Theilmeier G. (2013) Infection-associated platelet dysfunction of canine platelets detected in a flow chamber model. BMC Vet Res. 2013 Jun 7;9(1):112 (PMID: 23758817)
2. Ferkau A, Ecklebe S, Jahn K, Calmer S, Theilmeier G, Mischke R. (2013) A dynamic flow-chamber-based adhesion assay to assess canine platelet-matrix interactions in vitro. Vet Clin Pathol. 2013 Jun;42(2):150-6 (PMID: 23627964)
