342 Collagen type I (mouse) 1 mg

In Stock

195 USD

Article Number: 342

Murine collagen type I purified from mouse tail tendon


Source

Mouse tail tendon


Purity

Murine collagens: type I 90%, type III 10%, other collagen types below 1%. Non-collagen proteins below 0.5%


Format

Lyophilized, salt-free


Purification

Partial pepsin digestion in acidic conditions and differential salt precipitation


Applications

Coating material for cell culture studies. May not be suitable for 3-D gel formation


Reconstitution

Use 0.5 M acetic acid, pH 2.5 at 4°C. Collagen solubility 10 mg/ml. Dissolved collagen retains immunologic properties of native collagen. Structure of native collagen confirmed by ability to form microfibrils


Storage

Shipping at ambient temperature. Storage of lyophilized protein 2 years at -20°C. Storage of reconstituted protein 1 month at +4°C