634 Collagen type I and III (bovine) 10 mg

In Stock

115 USD

Article Number: 634

Bovine collagen type I and III protein purified from calf skin


Source

Bovine (calf) skin. Collagen was extracted from washed dissected tissue into dilute acetic acid after pepsin treatment. Collagen type I and III was purified by using differential salt precipitation


Purity

Bovine collagen type I 45%, bovine collagen type III 45%, bovine collagen other types <10%, non-collagen proteins <0.5%


Format

Lyophilized, salt-free


Purification

Partial pepsin digestion in acidic conditions and differential salt precipitation


Applications

Coating material for cell culture studies. May not be suitable for 3-D gel formation


Reconstitution

Use 0.5 M acetic acid, pH 2.5. Dissolved collagen retains immunologic properties of native collagen. Structure of native collagen confirmed by ability to form microfibrils


Storage

Shipping at ambient temperature. Storage of lyophilized protein 2 years at -20°C. Storage of reconstituted protein 1 month at +4°C. Do not freeze reconstituted collagen