785 Collagen type I/III (porcine, atelocollagen suitable for 3D gel, sterile, lyophilized)
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455 EUR
Porcine collagen type I/III (atelocollagen) for 3D gels and cell-culture coating
Mixed collagen type I and type III (about 90:10) prepared from porcine tissue by pepsin solubilisation, so the antigenic telopeptides are removed. Supplied sterile, lyophilised and salt-free, 25 mg per vial. The I/III blend mimics the composition of soft connective tissue and forms a fibrillar hydrogel on neutralisation, making it suitable for 3D cell culture, co-culture models and tissue-engineering scaffolds as well as for coating culture plastic.
Catalog Number 785
Amount 25 mg
Source
Porcine tissue
Purity
Porcine collagen type I > 90%, porcine collagen type III < 10%. Tested for sterility
Format
Lyophilized, salt-free
Purification
Collagen was extracted from washed dissected tissue with pepsin and dilute acetic acid. Collagen type I and III was purified by using differential salt precipitation
Applications
Coating material for cell culture studies (tested on human HepG2, primary rabbit corneal keratinocytes, rabbit corneal endothelial and epithelial cells, mouse embryonal fibroblasts, mouse peritoneal macrophages). Suitable for 3-D gel formation
Reconstitution
Acetic acid 10-50mM can be used for dissolving collagen to a concentration of 1-5 mg/ml overnight using gentle mixing at +4°C
Storage
Shipping at ambient temperature. Storage of lyophilized protein at -20°C. Storage of reconstituted protein 1 month at +4°C
RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, DRUG OR HOUSEHOLD USE
