631 Collagen type I (bovine) 10 mg

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151 EUR

Article Number: 631

Collagen type I protein purified from bovine skin


Source

Bovine (calf) skin. Collagen was extracted from washed dissected tissue into dilute acetic acid after pepsin treatment. Collagen type I was purified by using differential salt precipitation


Purity

Bovine collagen type I 90%. Other non-collagen proteins below 0.5%. Structure of native collagen was confirmed by ability to form microfibrils


Format

Lyophilized


Purification

Partial pepsin digestion in acidic conditions and differential salt precipitation


Applications

Standard for immunological assays. Antigen for antibody production. Coating material for cell culture studies. May not be suitable for 3-D gel formation


Reconstitution

Use 0.1 M acetic acid, pH 3.0


Storage

Lyophilized protein: shipping at ambient temperature, long term storage (2 years) at -20°C or lower. Storage of reconstituted protein 1 month at +4°C