631 Collagen type I (bovine) 10 mg
0 EUR
179 EUR
Bovine collagen type I, pepsin-solubilised from calf skin, lyophilised, 10 mg
Native, atelo-collagen type I purified from calf skin by pepsin digestion and differential salt precipitation, 90 % type I with less than 0.5 % non-collagen protein and retained ability to form microfibrils. Lyophilised, 10 mg; dissolves in 0.1 M acetic acid. Used as an immunoassay standard, immunogen and cell-culture coating; the mixed bovine type I + III preparation is listed as 634.
Source
Bovine (calf) skin. Collagen was extracted from washed dissected tissue into dilute acetic acid after pepsin treatment. Collagen type I was purified by using differential salt precipitation
Purity
Bovine collagen type I 90%. Other non-collagen proteins below 0.5%. Structure of native collagen was confirmed by ability to form microfibrils
Format
Lyophilized
Purification
Partial pepsin digestion in acidic conditions and differential salt precipitation
Applications
Standard for immunological assays. Antigen for antibody production. Coating material for cell culture studies. May not be suitable for 3-D gel formation
Reconstitution
Use 0.1 M acetic acid, pH 3.0
Storage
Lyophilized protein: shipping at ambient temperature, long term storage (2 years) at -20°C or lower. Storage of reconstituted protein 1 month at +4°C
