351 Collagen type I (rat) 20 mg

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195 EUR

Article Number: 351

Note: There is a later version of this product #602 with superior qualities that we recommend instead (click here)

Rat collagen type I


Rat tail tendon. Collagen was extracted from washed dissected tissue into dilute acetic acid after pepsin treatment. Collagen type I was purified by using differential salt precipitation


Rat collagen type I 90%. Rat collagen type III 10%. Other collagens below 1%. Non-collagen proteins below 0.5%


Lyophilized, salt-free


Partial pepsin digestion in acidic conditions and differential salt precipitation


Rat collagen (type I) standard. Antigen for antibody production. Coating material for cell culture studies. Collagen microfibrils will activate platelets causing aggregation. May not be suitable for 3-D gel formation


Use 0.5 M acetic acid, pH 2.5. Dissolved collagen retains immunologic properties of native collagen. Structure of native collagen confirmed by ability to form microfibrils


Shipping at ambient temperature. Storage 2 years at -20°C or lower


1. O'Brien K, Bhatia A, Tsen F, Chen M, Wong AK, et al. (2014) Identification of the Critical Therapeutic Entity in Secreted Hsp90a That Promotes Wound Healing in Newly ReStandardized Healthy and Diabetic Pig Models. PLoS ONE 9(12): e113956. doi:10.1371/ journal.pone.0113956 (use in pig cell migration assay) PMID: 25464502