351 Collagen type I (rat) 20 mg

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195 USD

Article Number: 351

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Rat collagen type I


Source

Rat tail tendon. Collagen was extracted from washed dissected tissue into dilute acetic acid after pepsin treatment. Collagen type I was purified by using differential salt precipitation


Purity

Rat collagen type I 90%. Rat collagen type III 10%. Other collagens below 1%. Non-collagen proteins below 0.5%


Format

Lyophilized, salt-free


Purification

Partial pepsin digestion in acidic conditions and differential salt precipitation


Applications

Rat collagen (type I) standard. Antigen for antibody production. Coating material for cell culture studies. Collagen microfibrils will activate platelets causing aggregation. May not be suitable for 3-D gel formation


Reconstitution

Use 0.5 M acetic acid, pH 2.5. Dissolved collagen retains immunologic properties of native collagen. Structure of native collagen confirmed by ability to form microfibrils


Storage

Shipping at ambient temperature. Storage 2 years at -20°C or lower


Citations

1. O'Brien K, Bhatia A, Tsen F, Chen M, Wong AK, et al. (2014) Identification of the Critical Therapeutic Entity in Secreted Hsp90a That Promotes Wound Healing in Newly ReStandardized Healthy and Diabetic Pig Models. PLoS ONE 9(12): e113956. doi:10.1371/ journal.pone.0113956 (use in pig cell migration assay) PMID: 25464502