176 Collagen type I (mouse, native collagen suitable for 3D gel, sterile, lyophilized)
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Article Number: 176
Mouse collagen type I, native, acid-soluble, sterile, lyophilised – for 3D gels, 1 mg
Native, non-pepsinised collagen type I extracted from mouse tail tendon in dilute acetic acid and purified by differential salt precipitation, with telopeptides intact so that it forms fibrillar 3D gels on neutralisation. Sterile, salt-free lyophilisate, 1 mg, dissolved in 20 mM acetic acid at 1–5 mg/ml. For 3D culture, organoid and invasion matrices, coating and as a species-matched standard in mouse models; the rat tail tendon equivalent is listed as 437.
Catalog Number 176
Amount 1 mg
Source
Mouse tail tendons
Purity
Mouse collagen type I > 90%, mouse collagen type III < 10%
Format
Lyophilized, salt-free
Purification
Collagen was extracted from washed dissected tissue into dilute acetic acid. Collagen type I was purified by using differential salt precipitation
Sterility
Passed sterility test for bacteria and fungi
Applications
Cell culture (coating or 3D gel)
Reconstitution
Dissolve in 20 mM acetic acid. Suspension should be shaken or stirred for several hours or overnight between 2-8°C. Material is dissolved when it appears homogeneous, with no schlieren pattern (light refraction due to differing protein concentrations in the suspension). Recommended concentration 1-5 mg/ml. After dissolution store 1 month at +4°C
Storage
Lyophilized protein: shipping at ambient temperature, long term storage (2 years) at -20°C or lower. Storage of reconstituted protein 1 month at +4°C
RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, DRUG OR HOUSEHOLD USE
