840 Urokinase human 20 ug
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225 EUR
Recombinant human urokinase (uPA, urokinase-type plasminogen activator, PLAU), 20 µg
Recombinant human urokinase-type plasminogen activator expressed in E. coli and purified by ion-exchange and immunoaffinity chromatography, 95 % pure at 54 kDa with a specific activity of 100 000 IU/mg. Lyophilised, 20 µg. An active serine protease (EC 3.4.21.73) for plasminogen activation and fibrinolysis assays, as an antigen and as a ligand for affinity chromatography.
Source
Recombinant. E. coli expression
Purity
Purity 95% at 54 kDa SDS-PAGE. Specific activity 100000 IU/mg. Lysis of fibrin thin layer gel by Astrup. Lysis of chromogenic substrate S-2225
Format
Lyophilized from 20 ml solution containing sodium phosphate 10 mM pH 7.5, sodium chloride 150 mM, urokinase 1 mg/ml
Purification
Ion-exchange chromatography and affinity chromatography on immobilized anti-urokinase monoclonal antibody
Applications
Antigen. Ligand for affinity chromatography. Enzyme, plasminogen activator
Reconstitution
Dissolve in distilled water
Storage
Shipping at ambient temperature. Storage 2 years at -20°C or lower
RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, DRUG OR HOUSEHOLD USE