534 Mouse anti-human IgG monoclonal antibody, clone EFE-565 1 ml
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363 EUR
Mouse anti-human IgG (gamma chain) monoclonal antibody, clone EFE-565, 1 ml
Mouse IgG1 monoclonal antibody specific for the gamma heavy chain of human IgG, with no cross-reaction to human IgA, IgE, IgM or other plasma proteins. Purified IgG fraction, lyophilised, 1 ml after reconstitution. A defined, lot-consistent alternative to polyclonal anti-human IgG for Western blot, ELISA and immunohistochemistry; the polyclonal rabbit anti-human IgG is 493.
Specificity
Human immunoglobulin IgG (gamma chain). No cross-reaction to human IgA, IgE, IgM or other human plasma proteins
Preparation
Precipitation with sodium sulphate and DEAE-cellulose chromatography
Format
Lyophilized from 0.01M sodium phosphate, 0.01M sodium borate, 0.15M sodium chloride, 1% dextran, 1% mannitol, pH 8
Applications and recommended dilutions
Western blotting, ELISA, Immunohistochemistry
Reconstitution
Use 1 ml sterile pure water
Storage
Shipping at ambient temperature. Storage of lyophilized antibodies at -20°C or lower. Avoid multiple freeze-thaw cycles
