287 Rabbit anti-human collagen type I polyclonal antibody 1 ml
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520 EUR
Rabbit anti-human collagen type I (COL1A1) polyclonal antibody, affinity-purified and cross-adsorbed, 1 ml
Rabbit antibody against native human collagen type I, purified on immobilised collagen I and cross-adsorbed against human plasma proteins and collagens II, III, IV and V for type specificity. Lyophilised, 1 ml after reconstitution. For ELISA, immunohistochemistry on frozen sections and ECM staining in fibroblast or osteoblast culture; the goat and monoclonal alternatives are 762 and 393, and the antigen is 130.
Specificity
Binds human Collagen type I. No cross-reactivity to human plasma proteins and human collagen types II, III, IV and V
Preparation
Affinity purification on immobilized collagen type I with cross-absorption to immobilised human blood plasma proteins and human collagen type II, III, IV and V
Format
Lyophilized from 0.01M sodium phosphate, 0.01M sodium borate, 0.15M sodium chloride, 1% dextran, 1% mannitol, pH 8
Applications and recommended dilutions
Detection of human collagen type I by ELISA (x3000), Histochemistry (frozen sections) (x20). Immunostaining of ECM in cell culture. Not tested for use on paraffin section
Reconstitution
Use 1 ml sterile pure water
Storage
Shipping at ambient temperature. Storage of lyophilized antibodies 2 years at -20°C or lower
