357 Integrin aVb3 (human) 10 ug
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Human integrin αVβ3 (vitronectin receptor, CD51/CD61), native heterodimer, 10 µg
Native αVβ3 integrin purified from human placenta by immunoaffinity chromatography and lyophilised, 10 µg. The intact αV (145 kDa) / β3 (92 kDa) heterodimer retains its ligand-binding conformation, so it serves as a receptor in solid-phase binding assays with vitronectin, fibronectin, fibrinogen, osteopontin and RGD peptides, as a positive control and standard in Western blotting, as an immunogen and as a target for screening αVβ3 antagonists in angiogenesis, tumour-invasion and osteoclast research. Also known as ITGAV/ITGB3, VNR, vitronectin integrin.
Source
Human placenta. Product was tested and found negative for HIV and Hepatitis B
Purity
SDS-PAGE with silver staining at least 95%. Two major bands corresponding to aV (145 kDa) and b3 (92 kDa) subunits (non-reduced conditions). A minor band (170 kDa) corresponds to b3 subunit disulphide dimer.
Format
Lyophilized from solution containing: 0.21 mg/ml aVb3, 20 mM Tris-HCl pH 7.5, 150 mM NaCl, 2 mM MgCl2, 0.2% Triton X-100
Purification
Affinity chromatography with immobilized monoclonal anti-aVb3 antibodies. A tissue detergent extract, which was applied to the column was prepared as described in (Belkin et al. 1990, J. Cell. Biol. 111:2159-2170)
Applications
Integrin aVb3 interacts with vitronectin, von Willebrand factor, fibronectin and fibrinogen in RGD dependent ELISA test. This format of aVb3 is appropriate for functional evaluation.
- Standard for electrophoresis, immunoblotting and ligand binding
- In vitro binding experiments with metalloproteinase MMP-2
Reconstitution
Water. Keep reconstituted protein at -70°C. Avoid repeated freeze-thaw cycles
Storage
Lyophilized product: shipping at ambient temperature, long-term storage at -20°C. Reconstituted protein: 1 year at -70°C
Reference
Wirkner M, Weis S, San Miguel V, lvarez M, Gropeanu RA, Salierno M, Sartoris A, Unger RE, Kirkpatrick CJ, del Campo A. (2011) Photoactivatable caged cyclic RGD peptide for triggering integrin binding and cell adhesion to surfaces. Chembiochem. 12(17):2623-9
Zhang B, Song W, Pang P, Zhao Y, Zhang P, Csabai I, Vattay G and Lindsay S (2017) Observation of giant conductance fluctuations in a protein. Nano Futures, Volume 1, Number 3
