638 Integrin a5b1 (human) 5 ug
0 EUR
179 EUR
Human integrin α5β1 (VLA-5, CD49e/CD29), native fibronectin receptor, 5 µg
Native heterodimeric integrin α5β1, the classical fibronectin receptor that binds the RGD and synergy sites of fibronectin and drives fibronectin fibrillogenesis, purified by affinity chromatography from virus-screened human placenta to at least 95 % purity. Supplied lyophilised from a Triton X-100 buffer with Mg²⁺, 5 µg. For electrophoresis and immunoblot standards and for ligand-binding assays with fibronectin (663) or its 120 kDa cell-binding fragment (175).
Source
Human placenta. Product was tested and found negative for HIV and Hepatitis B
Purity
SDS-PAGE with silver staining at least 95%. Two major bands corresponding to a5 (155 kDa) and b1 (110 kDa) subunits (non-reduced conditions).
Format
Lyophilized from solution containing: 0.26 mg/ml a5b1, 20 mM Tris-HCl pH 7.5, 150 mM NaCl, 2 mM MgCl2, 0.2% Triton X-100
Purification
Affinity chromatography. A tissue detergent extract, which was applied to the column was prepared as described in (Belkin et al. 1990, J. Cell. Biol. 111:2159-2170)
Applications
Standard for electrophoresis, immunoblotting and ligand binding
Reconstitution
Water. Keep reconstituted protein at -70°C. Avoid repeated freeze-thaw cycles
Storage
Lyophilized product: shipping at ambient temperature, long-term storage at -20°C. Reconstituted protein: 1 year at -70°C
