126 Integrin a1b1 (human) 5 ug

In Stock

115 USD

Article Number: 126

Human integrin a1b1, affinity purified, lyophilized (VLA-1, CD49a/CD29, ITGA1)


Source

Human smooth muscles. Product was tested and found negative for HIV and Hepatitis B


Purity

SDS-PAGE with silver staining at least 95%. Two major bands corresponding to a1 (195 kDa) and b1 (120 kDa) subunits (non-reduced conditions). A minor band (210 kDa) corresponds to b1 subunit disulphide dimer.


Format

Lyophilized from solution containing: 0.28 mg/ml a1b1, 20 mM Tris-HCl pH 7.5, 150 mM NaCl, 2 mM MgCl2, 0.2% Triton X-100


Purification

Affinity chromatography. A tissue detergent extract, which was applied to the column was prepared as described in (Belkin et al. 1990, J. Cell. Biol. 111:2159-2170)


Applications

Integrin a1b1 interacts with collagen I, IV and laminin in ELISA. This format of a1b1 is appropriate for functional evaluation


Reconstitution

Water. Keep reconstituted protein at -70°C. Avoid repeated freeze-thaw cycles


Storage

Lyophilized product: shipping at ambient temperature, long-term storage at -20°C. Reconstituted protein: 1 year at -70°C