336 Collagen type I (rat, native collagen suitable for 3D gel, sterile, lyophilized)

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Article Number: 336
Description
Collagen type I protein purified from rat tail tendon (native collagen suitable for making 3D gel). Sterile and lyophilized

Catalog Number 336
Amount 30 mg

Source
Rat tail tendons

Purity
Rat collagen type I > 90%, rat collagen type III < 10%

Format
Lyophilized, salt-free

Purification
Collagen was extracted from washed dissected tissue into dilute acetic acid. Collagen type I was purified by using differential  salt  precipitation

Sterility
Passed sterility test for bacteria and fungi

Applications
Cell culture (coating or 3D gel)

Reconstitution
Dissolve in 20 mM acetic acid. Suspension should be shaken or stirred for several hours or overnight between 2-8°C. Material is dissolved when it appears homogeneous, with no schlieren pattern (light refraction due to differing protein concentrations in the suspension). Recommended concentration 1-5 mg/ml. After dissolution store 1 month at +4°C

Storage
Lyophilized protein: shipping at ambient temperature, long term storage (2 years) at -20°C or lower. Storage of reconstituted protein 1 month at +4°C

RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, DRUG OR HOUSEHOLD USE