650 Apolipoprotein B (human) 0.5 mg

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315 USD

Article Number: 650

Apolipoprotein B protein (low density lipoproteins LDL) purified from human plasma


Source

Human plasma. Donors tested negative for HBsAg, HBc, HIV-I, HIV-II and HCV antibodies


Purity

At least 90% pure, judged by SDS-PAGE. Band at 550 kDa


Format

Solution containing 50 mM Na2CO3, pH 10, 50 mM NaCl and deozycholate. Non-sterile


Purification

Ultracentrifugation in density gradient. Delipidation with diethyl ether and ethanol. Gel filtration on Sephacryl S-300


Applications

Apolipoprotein B standard. Antigen for immunisation (1) antibody production. ELISA


Reconstitution

Sterile pure water or PBS


Storage

Storage of reconstituted product 1 month at +4°C, 1 year at -20°C or lower. Avoid multiple freeze-thaw cycles


Citations

1. Engelbertsen D, Rattik S, Knutsson A, Björkbacka H, Bengtsson E, Nilsson J. Induction of T helper 2 responses against human apolipoprotein B100 does not affect atherosclerosis in ApoE-/- mice. Cardiovasc Res. 2014 Jul 15;103(2):304-12. doi: 10.1093/cvr/cvu131. Epub 2014 May 27. PubMed PMID: 24866382