447 Mouse anti-human Apolipoprotein B monoclonal antibody, clone 12G10 1 ml
0 EUR
492 EUR
Mouse anti-human apolipoprotein B (ApoB-100) monoclonal antibody, clone 12G10, 1 ml
Mouse IgG1 monoclonal antibody recognising the N-terminal T4 fragment (residues 1–1297) of human apolipoprotein B, the protein component of LDL. Purified IgG fraction, lyophilised, 1 ml after reconstitution. Used for ELISA, immunohistology and capture of ApoB-containing lipoproteins from plasma; pairs with the polyclonal rabbit anti-ApoB antibody 843.
Specificity
Specific against T4 fragment (res. 1-1297), the N-terminal thrombolytic peptide of human Apolipoprotein B (LDL). No reactivity to other human plasma proteins
Preparation
Precipitation by sodium sulphate and anion-exchange chromatography (DEAE-cellulose) with gradient elution
Format
Lyophilized from 0.01M sodium phosphate, 0.01M sodium borate, 0.15M sodium chloride, 1% dextran, 1% mannitol, pH 8
Applications and recommended dilutions
Detection of Apolipoprotein B (LDL) in human plasma or serum
Extraction of Apo-B containing lipoproteins from human plasma
ELISA (x1000), Immunohistology (x100)
Reconstitution
Use 1 ml sterile pure water, aliquot and store at -20°C or lower.
Storage
Shipping at ambient temperature. Storage of lyophilized antibodies 2 years at -20°C or lower
