224 Collagen type III (rat) 10 mg

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Article Number: 224

Rat collagen type III


Source

Newborn rats. Collagen was extracted from washed dissected tissue into dilute acetic acid after pepsin treatment. Collagen type III was purified by using differential salt precipitation


Purity

Rat collagen type III 60%. Other rat collagens 40%. Non-collagen proteins below 0.5%


Format

Lyophilized, salt-free


Purification

Partial pepsin digestion in acidic conditions and differential salt precipitation


Applications

Rat collagen (type III) standard. Antigen for antibody production. Coating material for cell culture studies. Collagen microfibrils will activate platelets causing aggregation. May not be suitable for 3-D gel formation


Reconstitution

Use 0.5 M acetic acid, pH 2.5. Dissolved collagen retains immunologic properties of native collagen. Structure of native collagen confirmed by ability to form microfibrils


Storage

Lyophilized protein: shipping at ambient temperature, long term storage (2 years) at -20°C or lower. Storage of reconstituted protein 1 month at +4°C