175 Fibronectin 120 kDa cell attachment fragment (human) 0.5 mg
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317 EUR
Human fibronectin 120 kDa cell-binding fragment (RGD domain), 0.5 mg
Chymotryptic 120 kDa fragment of human plasma fibronectin spanning the central cell-binding domain, including the RGD sequence and synergy site recognised by integrins α5β1 and αVβ3, but lacking the gelatin/collagen- and heparin-binding regions. Purified from virus-screened plasma, lyophilised, 0.5 mg. A defined substrate for cell attachment, spreading and integrin-signalling assays; intact fibronectin is listed as 663.
Source
Human plasma. Negative for HBsAg, HCV, HIV1 and HIV2
Purity
One major band 120 kDa, no bands higher than 120 kDa
Format
Lyophilized from sodium citrate buffer pH 7.4
Purification
Chymotryptic digest of purified human plasma fibronectin. Affinity chromatography on gelatine agarose, heparin agarose and gel filtration
Applications
Cell attachment assays (promotes efficient adhesion and spreading of cells). Integrin signaling assays (stimulates integrin-dependent activation)
Reconstitution
Sterile pure water 0.5 ml. Incubate 15 min at 37°C. Stir gently. Do not vortex
Storage
Lyophilized protein: shipping at ambient temperature, storage (1 year) at -20°C or lower. Storage of reconstituted protein 6 months at -70°C. Avoid repeated freeze/thaw cycles
