175 Fibronectin 120 kDa cell attachment fragment (human) 0.5 mg

In Stock

135 USD

Article Number: 175

Description

Fibronectin 120 kDa fragment containing the cell attachment (RGD) site. Contains interaction sites for integrins alpha5 beta1 and alphaV beta3


Source

Human plasma. Negative for HBsAg, HCV, HIV1 and HIV2


Purity

One major band 120 kDa, no bands higher than 120 kDa


Format

Lyophilized from sodium citrate buffer pH 7.4


Purification

Chymotryptic digest of purified human plasma fibronectin. Affinity chromatography on gelatine agarose, heparin agarose and gel filtration


Applications

Cell attachment assays (promotes efficient adhesion and spreading of cells). Integrin signaling assays (stimulates integrin-dependent activation)


Reconstitution

Sterile pure water 0.5 ml. Incubate 15 min at 37°C. Stir gently. Do not vortex


Storage

Lyophilized protein: shipping at ambient temperature, storage (1 year) at -20°C or lower. Storage of reconstituted protein 6 months at -70°C. Avoid repeated freeze/thaw cycles