175 Fibronectin 120 kDa cell attachment fragment (human) 0.5 mg
0 EUR
225 EUR
Description
Fibronectin 120 kDa fragment containing the cell attachment (RGD) site. Contains interaction sites for integrins alpha5 beta1 and alphaV beta3
Source
Human plasma. Negative for HBsAg, HCV, HIV1 and HIV2
Purity
One major band 120 kDa, no bands higher than 120 kDa
Format
Lyophilized from sodium citrate buffer pH 7.4
Purification
Chymotryptic digest of purified human plasma fibronectin. Affinity chromatography on gelatine agarose, heparin agarose and gel filtration
Applications
Cell attachment assays (promotes efficient adhesion and spreading of cells). Integrin signaling assays (stimulates integrin-dependent activation)
Reconstitution
Sterile pure water 0.5 ml. Incubate 15 min at 37°C. Stir gently. Do not vortex
Storage
Lyophilized protein: shipping at ambient temperature, storage (1 year) at -20°C or lower. Storage of reconstituted protein 6 months at -70°C. Avoid repeated freeze/thaw cycles
