663 Fibronectin (human) 5 mg

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Article Number: 663

Fibronectin is a major protein found in human plasma that plays an important role in wound healing and embryonic development. The important property of fibronectin to mediate cell attachment is used in vitro to create more physiologically relevant surfaces for culturing cells. Addition of fibronectin is especially beneficial to promote cell attachment in serum-free media.

Fibronectin is a major protein found in human plasma and is purified by affinity chromatography. It is then lyophilized (from 2.5 ml of buffer (150 mM NaCl, 10 mM sodium phosphate pH 7.5) to ensure stability in storage and during shipping. Purity 95% (SDS-PAGE - double band 220 kDa)

Fibronectin is shipped at ambient temperature. Upon arrival store dry at -20°C or lower. Lyophilized product is moisture-sensitive. Reconstituted and sterile-filtered product can be stored for 1 month at +2...8°C

Reconstitution of lyophilized fibronectin is done with 5 ml of warm (37°C) PBS . Incubate at 37°C for 3 hours, occasionally inverting the vial. Do not vortex. Reconstituted solution contains 5 mg of soluble fibronectin and a minor quantity of insoluble fibronectin aggregate. Filter slowly through prewetted 0.2 um membrane filter. Concentration 1 mg/ml of fibronectin corresponds to A280 - 1.3

Amount 5 mg


Attachment of circulating angiogenic cell CACs (1)

Coating of polymer coverslips with fibronectin for attachment of HEK293.123 cells (2)

Attachment of pathogenic Escherichia coli and Salmonella through bacterial curli protein CsgA to fibronectin (3)

Adhesion of human dermal fibroblasts to fibronectin (4)

Fibronectin was used as a cell-adhesive factor on microstructured single-cell arrays of A549 and Huh7 cells (5)

Culture of human induced pluripotent stem cell-derived cardiomyocytes hiPSC-CMs on fibronectin-coated glass dishes (6)

Attachment and culture of MDCK cells on fibronectin-micropatterned surfaces (7)

Use of fibronectin for PAA gel cell culture of NIH-3T3 cells (8)

Cell adhesion for single-cell arrays of A549, HuH7, and MDA-MB-436 cell lines (9)

Cell adhesion protein for adhesion and growth of normal human dermal fibroblasts (NHDF) to create highly selective nanostructured surfaces (nanosphere lithography NSL) (10)

HUVEC cell culture (11)

Surface coating of a corneal implant made of collagen hydrogel to enhance the attachment and growth of human corneal epithelial cells, microcontact printing with fibronectin to promote cell adhesion and cell attachment, biointeractive scaffold to mimic the extracellular matrix (ECM) for regenerative medicine, lithographic patterning for cell attachment of corneal implants (12)

Cell culture on fibronectin-coated islands of pre-determined size and shape created by soft lithography on NHS-functionalized acrylamide (13)

Fibronectin was used for creating a pattern on substrate that was used for attachment and growth of induced pluripotent stem cell (iPSC)-derived cardiomyocytes (14)

Microcontact fibronectin printing on cytophobic polyacrylamide gels. Fibronectin forms a stable cell-adherent pattern for OS-HEK cell line (15)

Attachment of human dermal fibroblasts (16, 17)


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