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380 Mouse anti-human collagen type III monoclonal antibody, clone MC3-HA 1 ml

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492 EUR

Varenummer: 380
Mouse anti-human collagen type III monoclonal antibody, clone MC3-HA, 1 ml
Mouse monoclonal that recognises native human collagen type III with no cross-reactivity to the other collagen types or to plasma proteins. Purified from ascites by PEG precipitation and gel chromatography and lyophilised (reconstitute with 1 ml water). Used for ELISA (~1:1000) and immunohistology on frozen sections (~1:20) wherever type III collagen must be distinguished from type I — fibrosis, vascular and skin studies, intervertebral-disc research (see the publication below) and quality control of collagen preparations. Not suitable for denaturing conditions. Monoclonal partner to the polyclonal anti-collagen I/III cat. 198 and anti-collagen IV clone MC4-HA (cat. 533).

Specificity

Binds human Collagen type III. No cross-reactivity to other collagen types and human plasma proteins


Preparation

PEG-6000 precipitation and purification by gel chromatography


Format

Lyophilized from 0.01M sodium phosphate, 0.01M sodium borate, 0.15M sodium chloride, 1% dextran, 1% mannitol, pH 8

Applications and recommended dilutions

ELISA (x1000), Immunohistology (x20). Not suitable for denaturing conditions


Reconstitution

Use 1 ml sterile pure water


Storage

Shipping at ambient temperature. Storage of lyophilized antibody 2 years at -20°C or lower. Storage of reconstituted antibodies 10 days at +4°C


References

Capossela S, Schläfli P, Bertolo A, Janner T, Stadler BM, Pätzel T, Baur M, Stoyanov JV. (2014) Degenerated human intervertebral discs contain autoantibodies against extracellular matrix proteins. Eur Cell Mater. 27:251-63

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