898 Sheep anti-human IgM polyclonal antibody 1 mg
0 EUR
238 EUR
Sheep anti-human IgM polyclonal antibody, cross-adsorbed and affinity-purified, 1 mg
Sheep polyclonal antibody specific for human IgM, purified on immobilised human IgM and extensively cross-absorbed against human IgG, IgA, other plasma proteins and mouse immunoglobulins (IgA under 2 %, IgG under 1 %, mouse Ig under 1 % by ELISA). Supplied unconjugated at 2 mg/ml in 50 % glycerol, 1 mg. A high-titre mu-chain-specific secondary for IgM serology, Western blot, ELISA (1:30 000) and immunohistology; the goat and rabbit anti-human IgM are 321 and 818.
Specificity
Affinity measured in ELISA: human IgM 100%, human IgA <2%, human IgG <1%, human albumin <0.2%, murine IgG, IgM, IgA <1%
Preparation
Purification by affinity chromatography using immobilized human IgM. Antiserum was cross-absorbed with human IgG, IgA, other human plasma proteins and murine IgG, IgM, IgA
Format
Liquid formulation containing antibody (2 mg/ml) in 50% glycerol, 50 mM sodium phosphate, pH 7.5
Applications and recommended dilutions
Western blotting, ELISA (x30000), Immunohistology
Storage
Shipping at ambient temperature. Storage -20°C or lower
