130 Collagen type I (porcine, atelocollagen suitable for 3D gel, sterile, lyophilized)
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Article Number: 130
Porcine collagen type I (atelocollagen) for 3D collagen gels and coatings
Pepsin-solubilised collagen type I from porcine tail tendon, supplied sterile, lyophilised and salt-free (30 mg per vial). The atelocollagen dissolves in dilute acetic acid and, after neutralisation at 37°C, forms a fibrillar hydrogel for embedding cells or for coating culture plastic. Suitable for 3D cell culture, organoid and spheroid work and tissue-engineering scaffolds. Shipped from Sweden at ambient temperature.
Catalog Number 130
Amount 30 mg
Source
Porcine tail tendons
Purity
Porcine collagen type I ≥95%
Format
Lyophilized, salt-free
Purification
Collagen was extracted from washed dissected tissue into dilute acetic acid after mild pepsin treatment. Collagen type I was purified by differential salt precipitation
Sterility
Passed sterility test for bacteria and fungi
Applications
Cell culture: coating of culture surfaces, 3D collagen gel, scaffold preparation
Reconstitution
Dissolve in 10-50 mM sterile acetic acid. Stir the suspension gently for several hours or overnight at 4°C. The material is dissolved when it appears homogeneous, with no schlieren pattern (light refraction due to differing protein concentrations). Recommended concentration 1-5 mg/ml. After dissolution store up to 1 month at +4°C
Storage
Lyophilized protein: shipping at ambient temperature, long-term storage (2 years) at -20°C or lower. Reconstituted protein: 1 month at +4°C
RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, DRUG OR HOUSEHOLD USE
