130 Collagen type I (porcine, atelocollagen suitable for 3D gel, sterile, lyophilized)
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Article Number: 130
Description
Collagen type I protein purified from porcine tendon (atelocollagen suitable for making 3D gel). Sterile and lyophilized
Catalog Number 130
Amount 30 mg
Source
Porcine tail tendons
Purity
Porcine collagen type I 95%
Format
Lyophilized, salt-free
Purification
Collagen was extracted from washed dissected tissue into dilute acetic acid after mild pepsin treatment. Collagen type I was purified by using differential salt precipitation
Sterility
Passed sterility test for bacteria and fungi
Applications
Cell culture (coating or 3D gel)
Reconstitution
Dissolve in 10-50 mM sterile acetic acid. Suspension should be gently stirred for several hours or overnight at 4°C. Material is dissolved when it appears homogeneous, with no schlieren pattern (light refraction due to differing protein concentrations in the suspension). Recommended concentration 1-5 mg/ml. After dissolution store 1 month at +4C
Storage
Lyophilized protein: shipping at ambient temperature, long term storage (2 years) at -20°C or lower. Storage of reconstituted protein 1 month at +4°C
RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, DRUG OR HOUSEHOLD USE
