826-1 Collagen type II (bovine) 1 mg
0 EUR
391 EUR
Bovine collagen type II, 1 mg trial size – cartilage atelocollagen
The 1 mg pack of our pepsin-solubilised bovine type II collagen from knee-joint cartilage (≥95%, lyophilised salt-free). Convenient for use as a type II collagen standard in ELISA and Western blotting, as a positive control when characterising anti-collagen II antibodies, for small-scale coating in chondrocyte culture and for evaluating the material before ordering the 30 mg pack (cat. 826-30) used in collagen-induced arthritis protocols.
Catalog Number 826-1
Amount 1 mg
Source
Bovine knee joint. Collagen was extracted from washed dissected tissue into dilute acetic acid after pepsin treatment. Collagen type II was purified by using differential salt precipitation
Purity
Bovine collagen type II 95%. Other bovine collagen types below 5%. Non-collagen proteins below 0.5%
Format
Lyophilized, salt-free
Purification
Partial pepsin digestion in acidic conditions and differential salt precipitation
Applications
Bovine collagen (type II) standard.
Antigen for antibody production.
Coating material for cell culture studies.
Reconstitution
Add 50 mM acetic acid to collagen concentration 1-5 mg/ml. Mix vigorously overnight at +4°C
Storage
Lyophilized protein: shipping at ambient temperature, long term storage (2 years) at -20°C or lower. Storage of reconstituted protein 1 month at +4°C
