210 Collagen type II (human) 0.1 mg
0 EUR
345 EUR
Human collagen type II, native, pepsin-solubilised from cartilage, 0.1 mg
Native human collagen type II (COL2A1), the principal collagen of hyaline cartilage, purified from virus-screened human cartilage by pepsin digestion and differential salt precipitation to above 95 % type II. Lyophilised, 0.1 mg (100 µg); dissolves in 0.5 M acetic acid. Used as an immunoassay standard, immunogen (1), coating substrate for chondrocyte culture and antigen in arthritis research; the 1 mg pack is 882 and the matching goat antibody is 415.
Source
Human cartilage. Negative for HBsAg, HCV, HIV1 and HIV2
Purity
Human collagen type II > 95%, human collagen types I and III < 5%, other human collagen types and non-collagen proteins < 0.5%.
Format
Lyophilized
Purification
Partial pepsin digestion in acidic conditions and differential salt precipitation
Applications
Standard for immunological assays. Antigen for immunisation (1) and antibody production. Coating material for cell culture studies.
Reconstitution
Use 0.5 M acetic acid, pH 2.5
Storage
Shipping at ambient temperature. Storage 2 years at -20°C or lower
Citations
1. Gualandi L, Kamath R, Schwartz Sterman A (2014) Anti-collagen antibodies for treatment and diagnosis. US20170226196A1 patent application
2. Pal S, V. Badireenath Konkimalla (2016) Data on sulforaphane treatment mediated suppression of autoreactive, inflammatory M1 macrophages. Data in Brief. https://doi.org/10.1016/j.dib.2016.03.105
3. Rezaei N, Lyons A, Forde NR (2018) Environmentally controlled curvature of single collagen proteins. bioRxiv http://dx.doi.org/10.1101/279380
