206 Collagenase (Paralithodes Camtschatica) 1 mg

King crab collagenase (Paralithodes camtschaticus), cold-active collagenolytic protease complex, 1 mg
A complex of serine collagenolytic proteases (five bands, 18–34 kDa) from the hepatopancreas of the Kamchatka king crab, purified by affinity and ion-exchange chromatography and lyophilised. Being cold-adapted it digests collagen efficiently between 4 and 40 °C, making it a gentle alternative to bacterial collagenase for releasing cells from tissue and cultureware. Inhibited by DFP, PMSF and EDTA; 1 mg pack.

Source

King Crab (Paralithodes Camtschatica) hepatopancreases

Purity

Five bands in the range 18-34 kDa

Format

Lyophilized

Purification

Acetone extraction. Affinity and ion-exchange chromatography

Applications

Isolation of animal cells from tissues and cultureware. Use at pH 6-9, 4-40°C, 1-10mM CaCl2. Inhibition by DFP, PMSF and EDTA

Reconstitution

Dissolve in buffer containing 1-10 mM CaCl2 at pH 6-9 

Storage

Lyophilized protein: shipping at ambient temperature, long term storage (2 years) at -20°C or lower