141 Mouse anti-human Apolipoprotein E monoclonal antibody, clone 3D12 1 ml

In Stock

215 USD

Article Number: 141

Monoclonal antibody (clone 3D12, IgG1 fraction) anti-human Apolipoprotein E (APOE)

Specificity

Human Apolipoprotein E (all alleles). Reacts with LDL and VLDL. No cross-reactivity to other human plasma proteins


Preparation

Ammonium sulphate precipitation. DEAE-cellulose chromatography with gradient elution


Format

Lyophilised from solution containing 0.5 mg antibody, 1% mannitol, 0.15 M sodium chloride, 0.01 M sodium phosphate


Applications

Western blotting, ELISA (x1000), Immunohistochemistry (x100), IHC 1:25 (after deparaffinization, rehydration and treatment with pronase E ) (1)


Reconstitution

Use 1 ml sterile pure water


Storage

Shipping at ambient temperature. Storage of lyophilized antibodies 2 years at -20°C or lower


References

1) Klaver CCW, Kliffen M, van Duijn CM, Hofman A, Cruts M, Grobbee DE, van Broeckhoven C, de Jong PTVM (1998)  Genetic Association of Apolipoprotein E with Age-Related Macular Degeneration. Am. J. Hum. Genet. 63:200-206

2) Huter O, Wolf HJ, Schnetzer A, Pfaller K (1997) Lipoprotein lipase, LDL receptors and apo-lipoproteins in human fetal membranes at term. Placenta 18(8):707-715